Using 20x TE buffer make 1x solution. Components of the Quant-iT™ Picogreen™ dsDNA Assay Kit, including the Picogreen reagent and 100 µg/mL λDNA standard stock (Thermo Fisher Scientific), were utilized. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. io |. 100 ng/µL of Lamba (λ) DNA, sheared by intensive. 99 to $1999. Selectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 2. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). Showing 4 of 4. 3. dsDNA • Quant-iT PicoGreen dsDNA Assay Kit (Cat. No. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). 2. DNA standards were prepared from concentrated stock herring sperm dsDNA (Sigma, PN-A3294) in TE buffer (tris-EDTA, pH 7. no. Q33120). 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. 99 to $1999. 95 mL TE for the . Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 线，利用这一曲线并根据经验检测样本的浓度。Qubit dsDNA HS Assay可用于测量DNA浓度在10pg/µL 至100ng/µL 之间 的样本。超过此浓度范围的样本必须经过稀释然后进行重新 测试，以确保它们的结果落在参考标准的线性范围内。Qubit dsDNA HS Assay具有高度的准确. The Quant-iT™ Picogreen® double stranded DNA reagent is added and mixed with both the samples and references through a controlled aspiration and dispense. (An additional 1 ml TE with 5 ul PicoGreen may need to be prepared for the standards. 5 µL of each extracted sample was used for Picogreen analysis. Picogreen assays are compatible with most microplate readers and fluorometers. Picogreen assays are compatible with most microplate readers and fluorometers. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Quant-iT™ PicoGreen® dsDNA Assay KitLife TechnologiesCatalog #P7589 protocols. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile,Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo Fisher Scientific). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Unit Size. The libraries were sequenced using the Illumina HiSeq 2500. 9 Q33230 9 500 28. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). The Quant-iT dsDNA Broad-Range Kit is designed for assaying samples containing 2–1000 ng of DNA. No. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 995 mL TE for the . The micrococcal nuclease treatment used for fragmentation in some selected ChIP methods will generate. Catalog Number. PicoGreen®試薬アッセイ. (Left) The fluorescence signal from binding of the Quant-iT PicoGreen dsDNA Reagent to dsDNA is linear over at least four orders of magnitude; the inset shows the results obtained using 0–750 pg/ml calf thymus DNA. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Qubit assays are available for double- and single-stranded DNA (dsDNA and ssDNA), RNA, microRNA, and protein. 文件. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. The linear detection range of the PicoGreen® assay in the 20/20n extends over three orders of magnitude in DNA concentration - from 450 pg/mLFor DNA quantification with PicoGreen (PG), Quant-iT™ PicoGreen® dsDNA Reagents and Kits (Invitrogen – Molecular Probes, Eugene, OR, USA) were used. coli rRNA (Δ), or a 1:1 mixture of DNA and RNA (∎) were assayed in the Quant-iTTM dsDNA HS assay. The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). DNA standard curve. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. i) 110 sample x 150uL =16. PicoGreen is widely used in molecular biological procedures such as DNA fragment purification for subcloning, cDNA synthesis for library production as well as primer assays. Rapidly detect as little as 0. Rapidly detect as little as 0. Catalog Number. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Technical Support Customer Service. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. Skip to main list 美谷分子仪器（上海）有限公司. The assay is performed at room temperature, and the signal is stable for 3 hours. Quantitation Range. The assay is highly selective for double- The assay is highly selective for double- stranded DNA over RNA (see “Assay selectivity” on page 1), and in the range of 0. Because the PicoGreen dye is an extremely sensitive detection reagent for dsDNA, it is imperative that the TE buffer is free of contaminating nucleic acids. Pico488 allows to quantitate as little as low pg/mL of dsDNA. 2 The input amount of linear dsDNA library is based on the available linear dsDNA (see Table 3). The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. Rapidly detect as little as 0. Picogreen assays are compatible with most microplate readers and fluorometers. Fluorometric dsDNA Assay. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. the automated setup to the manual setup of the Quant-iT PicoGreen dsDNA Assay Kit. • Suitable for use in 96- and 384-well plate formats. Katalognummer: P11495. Facebook. This protocol is a simplified and condensed. This is Standard 1 which will have a final concentration of 1 ug/mL. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. This kit contains. 2023-11. The Qubit dsDNA HS and BR Assay Kits are accurate from 10 pg/L to 100 ng/uL, and 100 pg/L to 1,000 ng/L, respectively. 5 ng/µL. dsDNA만을 선택적으로 정량. The fluorescence intensity as a function of the amount of dsDNA is measured at the optimal. This was possible since, due to the low height of the light ray of only 8. 10. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Quant It. The tubes were. Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen) May 22, 2007. stranded DNA (dsDNA). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. Note: The 20X TE buffer (Component B) included in the PicoGreen dsDNA Quantitation Kit is certified to be nucleic acid-free and DNase-free. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:After completion of DNA extraction, double-stranded DNA (dsDNA) is quantified by the Quant-iT™ PicoGreen® fluorescence assay using the provided lambda DNA standards (Invitrogen) prior to Library Construction (LC). 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. , QuantIt PicoGreen dsDNA assay). Antibodies Recombinant antibodies, monoclonal & polyclonal antibodies and primary & secondary antibodies. Picogreen assays are compatible with most microplate readers and fluorometers. Development and characterization of a novel host cell DNA assay using ultra-sensitive fluorescent nucleic acid stain “PicoGreen. 10 ng/mkl – 10000 ng/ml. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. • Specific for dsDNA in the presence of RNA. P11496). Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されて. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. PicoGreen dsDNA quantitation reagents are. The following protocol is an adaptation of the Mo-lecular ProbesTM dsDNA Quantitation Reagent and Kits product information sheet. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). This protocol is a simplified and condensed. 1. • Suitable for use in 96- and 384-well plate formats. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 25 pg/uL 的 dsDNA。 该定量试剂盒在三个数量级内呈线性，几乎无序列依赖性，可让您准确测定多种来源的 DNA，包括基因组 DNA、病毒 DNA、小提 DNA 或 PCR 扩. PicoGreen Solution must be diluted fresh before each assay run. 0 fluorometer (Selection Guide for the Qubit and Quant-iT Assay Kits. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. AccuBlue® RNA Quantitation Kit Details. 50 pg to 2 µg. 2–400ng/µl (1µl sample in 200µl assay). a) 16. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. • Suitable for use in 96- and 384-well plate formats. quant it picogreen dsdna assay kit , 2023-10. The accuracy index is defined in Table 2. We quantified the extract with Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents (Thermos Fisher Scientific, Waltham, MA, USA) to evaluate the amount of residual DNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. high-range. For a standard. Fluorescence was measured at. 0 mL to 200 uL to fit a 96-well microplate format. Triplicate 10 µL samples of DNA (), Escherichia coli rRNA or a 1:1 mixture of DNA and RNA were assayed with the Quant-iT dsDNA High-Sensitivity Assay Kit (). Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. After a 5-minute. Quant-iT PicoGreen dsDNA Assay Kit a. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM製品番号（カタログ番号）： P11495. VIEWS. Product Type. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. 86 / 100 stars. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. for points 1–4 above) prior to cfDNA quantification can be determined for each quantification method and for a range of experimental scenarios for. Prepare the 1x TE buffer by diluting the 20x TE concentrate 1:20 by deionized water. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. For Research Use. The assay is highly selective for RNA over double-stranded DNA, and provides a dynamic range of 5-100 ng of RNA. 1 Prepare a 2 μ g/mL stock solution of dsDNA in TE. • Suitable for use in 96- and 384-well plate formats. is 1 pg/μL – 5 ng/μL so the sensitivity of this kit can be rated as Ultra High (UHS). Quant-iT dsDNA BR buffer (Component B) 250 ml c. Buy from Supplier. Picogreen Reagent b. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. In addition to the Quant-iT™ dsDNA Broad-Range Assay Kit described here, we also offer the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Cat. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. The high standard corresponds to the same concentration as the highest concentration dsDNA standard in the Quant-iT Assay Kits and can be diluted into the standard series. 1 mL kit. DNA content was then determined using the Quant-iT PicoGreen dsDNA assay kit per manufacturer’s instructions. Prepare the rest of the standards , to prepare enough working solution to assay 100 samples in 200 µL volumes, add 50 µL Quant-iT ™ RiboGreen RNA Reagent to 9. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. A Qubit dsDNA BR (broad range, 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. Calibrator DNA content was assessed with Quant-iT™ PicoGreen™ dsDNA Assay Kit (Thermo. Rapidly detect as little as 0. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). Quant-IT dsDNA Assay Kit, high sensitivity Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target: dsDNA: dsDNA: dsDNA: Quantitation range: 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The assay is linear over three orders of magnitude and has little sequence. Rapidly detect as little as 0. This assay is designed for use with fluorescence microplate readers. Three kits covering a wide range of dsDNA concentrations. • Suitable for use in 96- and 384-well plate formats. If your are using plastic cuvettes or assay plates, reduce this problem by including 0. Short-read sequencing was performed on an Illumina NextSeq 2000 platform (2x151bp. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays*Selection Guide for the Qubit and Quant-iT Assay Kits—Table 8. The Quant-iT 1X dsDNA BR Assay Kit is accurate for initial DNA sample concentrations of 200 pg/µL to 4000 ng/µL (depending on sample volume. Instead of 2 mL, only 1 mL measurement volume was used. 0 ml 200x in DMSO b. 作成者 LatB Staff 01. Table 2 summarizes results of the. 2023. The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and accurate. 5. Quant-iT dsDNA Assay Kit, broad range Quant-iT 1X dsDNA high sensitivity; Target. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. • Suitable for use in 96- and 384-well plate formats. P11495. Quantitation Range. No. 製品番号（カタログ番号）： P7581. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). 1 mL kit. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. Bioz Stars score: 99/100, based on 1 PubMed citations. • Specific for dsDNA in the presence of RNA. DNA standard curve. The Quant-iT 1X dsDNA HS Assay Kit is accurate for initial sample concentrations ranging from 10 pg/µL to 100 ng/µL, providing a core detection range of 0. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. Final libraries were quantified using a Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) on a SpectraMax iD3 (Molecular Devices). You will need 100 μl diluted PicoGreen reagent per well. If you would like to use this kit with the Qubit® fluorometer, we have includedQuant-iT ™ PicoGreen ™ dsDNA Reagent 1 mL reagent 10 x 100 µL P7581 P11495 TE Buffer (20X), RNase-free 100 mL T11493. pDNA encapsulation efficiency of LNP 10 and LNP 20 was measured by using a Quant-iT Pico-Green dsDNA assay kit (ThermoFisher Scientific, Waltham, MA, USA). Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. In this experiment we followed the instructions of the Quant-iT™ PicoGreen dsDNA Assay Kit to compare setup on the Matrix Hydra DT and Matrix PlateMate 2x2 to manual setup and then reduced the total volume from 210 µl to 105 µl on both instruments. 2 - 100 ng/ml 480/520 NaCl, MgCl 2Quant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Fluorometer. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. 2-200 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iTTM DNA Assay Kit, Broad Range 2-1000 ng/ml 480/520 NaCl, MgCl 2, possible mutagen - wear protective clothing Quant-iT™ DNA Assay Kit, High Sensitivity 0. The reagent must be completely thawed before using it by bringing it to room temperature. The limit of detection for the 20/20n is less than 45 pg in 100 µL total volume. 2 to 100 ng) Assay Kit were used with a Qubit 3. 10 x 100 µL. In addition, the assay failed to detect in vitro generated MPO-DNA complexes when spiked into plasma. The PicoGreen assay has high specificity for dsDNA versus RNA or ssDNA, and is. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. 0, 20X c. 4). The ITS protocol detailed here is designed to amplify fungal microbial eukaryotic lineages using paired-end community sequencing on the Illumina platform with primers ITS1f-ITS2 (EMP. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Price from $9. Catalog number: P7581. Prediluted standards provided with the Quant-iT assay were used to generate a standard curve. Picogreenアッセイは、ほとんどのマイクロプレートリーダーおよび蛍光光度計に. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 1 mL kit. 710 citations – Kit [P7589] Quant-iT™ PicoGreen™ dsDNA Assay Kit by Thermo Scientific. 3,201 3. Tris-EDTA Buffer (10 mM tris, 1 mM EDTA), pH8. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 3. 99 to $1999. Louis, MI, USA). Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Rapidly detect as little as 0. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA quantitation, measuring dsDNA in complex mixtures, and viral DNA quantitation. Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. Add-and-read format makes measuring low concentrations of dsDNA simple—no dilutions, no extra tubes. 50 pg to 2 µg. 86. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration. The QFX is a powerful solution for all fluorometric applications where enhanced specificity or sensitivity are needed. Rapidly detect as little as 0. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. PicoGreen dsDNA Assay Kit. 2–100 ng Quant-iT dsDNA HS Assay Kit 0. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. Average size fragment was determined using a LabChip GX (PerkinElmer) instrument. Spectral Data: Quant-iT™ PicoGreen® dsDNA reagent 502/523 nm, bound to nucleic acids Quant-iT™ PicoGreen ® dsDNA Reagent and KitsQuant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). Tweet. Picogreen Assays sind mit den meisten Mikrotiterplatten-Lesegeräten und Fluorometern kompatibel. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. Lambda DNA contained in the kit was used to create a six-point standard curve from 3. The Quant-iT™ PicoGreen® dsDNA Assay Kit makes for an easy, reliable, and accurate method to quantify dsDNA samples when used in conjunction with the Modulus™ Microplate Fluorometer from Turner BioSystems, Inc. Absorbance-based measurements employ the DNA as it is and volumes of 100 µl, 50 µl, and 2 µl were placed into a 96 well, 384 well, or LVis plate, respectively. 2–100 ng, the fluorescence signal is linear with DNA. Using this data as a starting point, the optimal conditions (e. Quant-iT PicoGreen dsDNA Assay Kit. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. 22. 7. Alternatively, purified RCA products can be quantitated by measuring the absorbance at 260 nm or by NanoDrop®. 95 mL TE for the . Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). All NGS libraries generated with both the Fragmentase and the FTP method contained similar amounts of ds DNA (800 ± 50 ng) and had similar mean insert sizes of the libraries in a range from 400 to 500 bp. When 5–500 pg salmon dsDNA or 3–1000 pg control dsDNA (calf thymus dsDNA standard) is added to AccuClear a linear response is observed (Fig. no. 5 ng/ml of dsDNA were detected with minimal effect in the presence of ssDNA, RNA, and free nucleotides, which is comparable with InvitrogenTM Quant-iT™ PicoGreen® dsDNA Reagent. 製品概要. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Genomic DNA was quantified using the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions. Q33130 Introduction The Quant-iT™ dsDNA Broad-Range Assay Kit makes DNA quantitation easy and. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 10 x 100 µL. no. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. For other nucleic acid methods (RiboGreen, OliGreen), quantified in thePicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Figure 1. Unit Size. Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. 5mL. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using the PicoGreen dsDNA quantitation assay and reagent. 激发/发射. Lambda DNA was used to generate the. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The DeNovix dsDNA High. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. クイックオーダー. Dilute from 20xTE stock (supplied in the PicoGreen kit). Each Helixyte™ assay kit is specially formulated with a target-selective DNA. Quant-iT Assay Kits Quant-iT DNA Assay Kit, High Sensitivity The Quant-iT High-Sensitivity DNA Assay Kit has a linear detection range of 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Unit Size. Rapidly detect as little as 0. ” application on Lunatic and compared to Qubit and. 50 pg to 2 µg. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Qubit Flex NGS Starter Kit 1 instrument kit A45924 Microplate assays and instruments Quant-iT PicoGreen dsDNA Assay Kit 1,000 assays P11496 Quant-iT dsDNA Assay Kit, high sensitivity 1,000 assays Q33120 Quant-iT dsDNA Assay Kit, broad range 1,000 assays Q33130 Quant-iT RiboGreen RNA Assay Kit 1,000 assays R11490Quant-iT™ PicoGreen ® dsDNA Kit (Invitrogen, #P11496). Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. 2. DNA was quantified by the Quant-it PicoGreen dsDNA Assay Kit (Invitrogen Life technologies, UK) according to the manufacturer’s protocol. HepG2 cell number standard curve determined using the PicoGreen® assay (Quant-iTTM PicoGreen® dsDNA reagent kit, Invitrogen), HepG2 cells were harvested from conventional 2D T75 culture flasks and counted using a haemocytometer and Trypan Blue dye exclusion. Picogreen assays are compatible with most microplate readers and fluorometers. PicoGreen dsDNA quantitation reagents are. 1. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesQuant-iT PicoGreen dsDNA Assay Kit (10 x 100 µL) Quant-iT PicoGreen dsDNA Reagent (10 x 100 µL) dsDNA : 50 pg–2 µg : 502/523 : 2,000 : P11496 P11495: Quant-iT OliGreen ssDNA Assay Kit Quant-iT OliGreen ssDNA Reagent: ssDNA : 200 pg–2 µg : 500/525 : 2,000 : O11492 O7582: Quant-iT RiboGreen RNA Assay Kit Quant-iT. Highly selective for dsDNA over ssDNA or RNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The Quant-iTTM PicoGreenTM dsDNA Reagent enables researchers to quantitate as little as 250 pg/mL of dsDNA (50 pg dsDNA in a 200 μL assay volume) with a fluorescence. Picogreen assays are compatible with most microplate readers and fluorometers. • Specific for dsDNA in the presence of RNA. Automation of the Quant-iT™ PicoGreen® dsDNA Assay Kit on the Fluent. assay. Figure 8. 005% Triton X-100, Tween-20, or Brij. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14.